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In our latest preprint, Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition, we introduce native electrospray ion-beam deposition (native ES-IBD), a mass-selective sample preparation workflow for cryo-EM. Folded protein ions are generated by native electrospray ionization, mass-filtered, and gently deposited on cryo-EM grids, which are subsequently frozen in liquid nitrogen. Native ES-IBD has the potential to complement and accelerate established cryo-EM sample preparation workflows, by direct correlation between cryo-EM maps with precise information on composition and conformation. We show 3D reconstructions of protein assemblies purified in the gas-phase.